Click image to see more details
Product Info Summary
| SKU: | FC05978-FITC |
|---|---|
| Size: | 100 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated
View all CEACAM8/CD66b Antibodies
SKU/Catalog Number
FC05978-FITC
Size
100 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC05978-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC05978-FITC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: G10F5
Isotype
IgM,k
Immunogen
Human Peripheral Blood Cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC05978-FITC is reactive to CEACAM8 in Human
Applications
FC05978-FITC is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38154 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Granulocytes gated RBC lysed blood stained with FITC conjugated anti-human CD66b (clone G10F5) (red histogram). Granulocytes gated RBC lysed blood stained with FITC conjugated mouse IgM isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For CEACAM8 (Source: Uniprot.org, NCBI)
Gene Name
CEACAM8
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 8
Weight
38154 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; cd66b clone g10f5; cd66b G10F5; cd66b granulocyte marker; cd66b ihc; cd66b marker; cd66b neutrophil marker; cd66b neutrophils; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95 CEACAM8 CD66b, CD67, CGM6, NCA-95 CEA cell adhesion molecule 8 carcinoembryonic antigen-related cell adhesion molecule 8|CD67 antigen|carcinoembryonic antigen CGM6|carcinoembryonic antigen gene family member 6|carcinoembryonic antigen related cell adhesion molecule 8|non-specific cross-reacting antigen NCA-95
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEACAM8, check out the CEACAM8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEACAM8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated (FC05978-FITC)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Human CD66b CEACAM8 Monoclonal Antibody FITC Conjugated, Flow Validated
Question
I have a question about product FC05978-FITC, anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC05978-FITC anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-06
Question
I was wanting to use your anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human bone marrow on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human bone marrow identification?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
As indicated on the product datasheet, FC05978-FITC anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human bone marrow in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-17
Question
I see that the anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated FC05978-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-15
Question
Is a blocking peptide available for product anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated (FC05978-FITC)?
J. Patel
Verified customer
Asked: 2017-02-22
Answer
We do provide the blocking peptide for product anti-Human CD66b Monoclonal antibody FITC Conjugated, Flow Validated (FC05978-FITC). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-22
