Anti-Hu CD300a Purified Monoclonal Antibody

Product Name

Anti-Hu CD300a Purified Monoclonal Antibody

View all CD300a/LMIR1 Antibodies

SKU/Catalog Number

M05953

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD300a Purified Monoclonal Antibody (Catalog# M05953). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD300a Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05953)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-260

Isotype

Mouse IgG1

Immunogen

HPB human acute lymphoid leukemia cell line. The antibody MEM-260 reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Cross-reactivity with CLIC5b was not determined.

Reactive Species

M05953 is reactive to CD300A in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

33201 MW

Background of CD300a/LMIR1

CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M05953 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 2 μg/ml.
Western blotting: Positive material: HPB cell line, negative material: JURKAT human cell line, RAJI human cell line; non-reducing conditions.

Validation Images & Assay Conditions

Gene/Protein Information For CD300A (Source: Uniprot.org, NCBI)

Alternative Names

IRC, CLM-8, IGSF12, CMRF35H CD300A CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60 CD300a molecule CMRF35-like molecule 8|CD300 -like family member A|CD300a |CMRF35H leukocyte immunoglobulin-like receptor|NK inhibitory receptor|immunoglobulin superfamily member 12|inhibitory receptor protein 60|leukocyte membrane

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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