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Product Info Summary
| SKU: | A04608-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HSS/SGSH Antibody Picoband®
View all Sulfamidase/SGSH Antibodies
SKU/Catalog Number
A04608-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSS/SGSH Antibody Picoband® catalog # A04608-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HSS/SGSH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04608-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HSS/SGSH recombinant protein (Position: R206-L502).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04608-1 is reactive to SGSH in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
56.695kDa
Background of Sulfamidase/SGSH
N-sulphoglucosamine sulphohydrolase is an enzyme that in humans is encoded by the SGSH gene. This gene encodes the enzyme sulfamidase; one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with the lysosomal storage disease mucopolysaccaridosis IIIA, also known as Sanfilippo syndrome A, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04608-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human MCF-7 whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSS/SGSH antigen affinity purified polyclonal antibody (Catalog # A04608-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSS/SGSH at approximately 70 kDa. The expected band size for HSS/SGSH is at 56 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SGSH (Source: Uniprot.org, NCBI)
Gene Name
SGSH
Full Name
N-sulphoglucosamine sulphohydrolase
Weight
56.695kDa
Superfamily
sulfatase family
Alternative Names
Thioredoxin, mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TXN2; TRX2 SGSH HSS, MPS3A, SFMD N-sulfoglucosamine sulfohydrolase N-sulphoglucosamine sulphohydrolase|heparan sulfate sulfatase|mucopolysaccharidosis type IIIA|sulfoglucosamine sulfamidase|sulphamidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGSH, check out the SGSH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGSH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSS/SGSH Antibody Picoband® (A04608-1)
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