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Product Info Summary
| SKU: | A14896 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HSP77/76 Antibody
SKU/Catalog Number
A14896
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSP77/76 Antibody catalog # A14896. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP77/76 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14896)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human HSP77/76.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14896 is reactive to HSPA7 in Human
Reconstitution
Calculated molecular weight
40244 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14896 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of K562 cells using HSP77/76 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HSPA7 (Source: Uniprot.org, NCBI)
Gene Name
HSPA7
Full Name
Putative heat shock 70 kDa protein 7
Weight
40244 MW
Superfamily
heat shock protein 70 family
Alternative Names
Putative heat shock 70 kDa protein 7;Heat shock 70 kDa protein B;HSPA7;HSP70B; HSPA7 HSP70B heat shock protein family A (Hsp70) member 7 (pseudogene) heat shock 70kD protein 7 (HSP70B)|heat shock 70kDa protein 7 (HSP70B)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA7, check out the HSPA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP77/76 Antibody (A14896)
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No publications found for A14896
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-HSP77/76 Antibody
Question
We are currently using anti-HSP77/76 antibody A14896 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-HSP77/76 antibody (A14896) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
I see that the anti-HSP77/76 antibody A14896 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-03
Question
Is there a BSA free version of anti-HSP77/76 antibody A14896 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HSP77/76 antibody A14896 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-HSP77/76 antibody (A14896)?
K. Miller
Verified customer
Asked: 2018-08-10
Answer
We do provide the blocking peptide for product anti-HSP77/76 antibody (A14896). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-10
Question
Is this A14896 anti-HSP77/76 antibody reactive to the isotypes of HSPA7?
M. Parker
Verified customer
Asked: 2017-07-04
Answer
The immunogen of A14896 anti-HSP77/76 antibody is Synthesized peptide derived from the Internal region of human HSP77/76. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-04
Question
Will A14896 anti-HSP77/76 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Johnson
Verified customer
Asked: 2016-03-11
Answer
It shows on the product datasheet, A14896 anti-HSP77/76 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-11
