Anti-HSP77/76 Antibody

HSPA7 antibody

Boster Bio Anti-HSP77/76 Antibody catalog # A14896. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A14896
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-HSP77/76 Antibody

View all HSPA7 Antibodies

SKU/Catalog Number

A14896

Size

100μl

Form

Liquid

Description

Boster Bio Anti-HSP77/76 Antibody catalog # A14896. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSP77/76 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14896)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human HSP77/76.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14896 is reactive to HSPA7 in Human

Applications

A14896 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40244 MW

Background of HSPA7

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For HSPA7 (Source: Uniprot.org, NCBI)

Gene Name

HSPA7

Full Name

Putative heat shock 70 kDa protein 7

Weight

40244 MW

Superfamily

heat shock protein 70 family

Alternative Names

Putative heat shock 70 kDa protein 7 HSPA7 HSP70B heat shock protein family A (Hsp70) member 7 (pseudogene) heat shock 70kD protein 7 (HSP70B)|heat shock 70kDa protein 7 (HSP70B)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPA7, check out the HSPA7 Infographic

HSPA7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-HSP77/76 Antibody

Question

We are currently using anti-HSP77/76 antibody A14896 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The anti-HSP77/76 antibody (A14896) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-16

Question

I see that the anti-HSP77/76 antibody A14896 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-03

Question

Is there a BSA free version of anti-HSP77/76 antibody A14896 available?

Verified Customer

Verified customer

Asked: 2019-01-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HSP77/76 antibody A14896 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-23

Question

Is a blocking peptide available for product anti-HSP77/76 antibody (A14896)?

K. Miller

Verified customer

Asked: 2018-08-10

Answer

We do provide the blocking peptide for product anti-HSP77/76 antibody (A14896). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-10

Question

Is this A14896 anti-HSP77/76 antibody reactive to the isotypes of HSPA7?

M. Parker

Verified customer

Asked: 2017-07-04

Answer

The immunogen of A14896 anti-HSP77/76 antibody is Synthesized peptide derived from the Internal region of human HSP77/76. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-07-04

Question

Will A14896 anti-HSP77/76 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Johnson

Verified customer

Asked: 2016-03-11

Answer

It shows on the product datasheet, A14896 anti-HSP77/76 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-03-11

Order DetailsPrice
A14896

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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