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Product Info Summary
| SKU: | M06045 |
|---|---|
| Size: | 100 µl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HoxC6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06045
Size
100 µl/vial
Form
Liquid
Description
Boster Bio Anti-HoxC6 Rabbit Monoclonal Antibody catalog # M06045. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HoxC6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06045)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
31H41
Isotype
IgG
Immunogen
A synthesized peptide derived from human HoxC6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06045 is reactive to HOXC6 in Human, Mouse, Rat
Applications
M06045 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
26,27 kDa
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000 ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HoxC6 using anti-HoxC6 antibody (M06045).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HoxC6 antigen affinity purified monoclonal antibody (Catalog # M06045) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HoxC6 at approximately 26,27 kDa. The expected band size for HoxC6 is at 27 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For HOXC6 (Source: Uniprot.org, NCBI)
Gene Name
HOXC6
Full Name
Homeobox protein Hox-C6
Weight
Superfamily
Antp homeobox family
Alternative Names
CP25; HHO.C8; homeo box 3C; homeo box C6; homeo box C8 protein; homeobox C6; Homeobox protein CP25; Homeobox protein HHO.C8; Homeobox protein Hox-3C; homeobox protein Hox-C6; HOX3CHOX3 HOXC6 CP25, HHO.C8, HOX3, HOX3C homeobox C6 homeobox protein Hox-C6|homeo box 3C|homeo box C8 protein|homeobox protein CP25|homeobox protein HHO.C8|homeobox protein Hox-3C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXC6, check out the HOXC6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HoxC6 Rabbit Monoclonal Antibody (M06045)
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