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Product Info Summary
| SKU: | A01783-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Hox8/MSX2 Antibody Picoband®
SKU/Catalog Number
A01783-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hox8/MSX2 Antibody Picoband® catalog # A01783-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Hox8/MSX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01783-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Hox8/MSX2 recombinant protein (Position: M1-M129).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01783-2 is reactive to MSX2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
57862 MW
Background of MSX2
Homeobox protein MSX-2 is a protein that in humans is encoded by the MSX2 gene. This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01783-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human MCF-7 whole cell, human T-47D whole cell
FCM: PC-3 cell, RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Hox8/MSX2 using anti-Hox8/MSX2 antibody (A01783-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hox8/MSX2 antigen affinity purified polyclonal antibody (Catalog # A01783-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hox8/MSX2 at approximately 37 kDa. The expected band size for Hox8/MSX2 is at 29 kDa.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-Hox8/MSX2 antibody (A01783-2).
Overlay histogram showing PC-3 cells stained with A01783-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hox8/MSX2 Antibody (A01783-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of RT4 cells using anti-Hox8/MSX2 antibody (A01783-2).
Overlay histogram showing RT4 cells stained with A01783-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hox8/MSX2 Antibody (A01783-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MSX2 (Source: Uniprot.org, NCBI)
Gene Name
MSX2
Full Name
Homeobox protein MSX-2
Weight
57862 MW
Superfamily
Msh homeobox family
Alternative Names
Farnesyl pyrophosphate synthase; FPP synthase; FPS; (2E,6E)-farnesyl diphosphate synthase; Cholesterol-regulated 39 kDa protein; CR 39; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Geranyltranstransferase; Fdps MSX2 CRS2, FPP, HOX8, MSH, PFM, PFM1 msh homeobox 2 homeobox protein MSX-2|homeobox protein Hox-8|msh homeo box 2|msh homeobox homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSX2, check out the MSX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hox8/MSX2 Antibody Picoband® (A01783-2)
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