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Product Info Summary
| SKU: | M00241-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214
SKU/Catalog Number
M00241-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (Catalog # M00241-2). Tested in WB, ICC. ELISA, Luminex applications. This antibody reacts with Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00241-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM214
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross-reactivity with other histone proteins. .
Reactive Species
M00241-2 is reactive to H2AFX in Vertebrates
Reconstitution
Calculated molecular weight
15145 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00241-2 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 ug/mL – 2 ug/mL
ICC: 1 ug/mL - 2 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.
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Figure 2. ICC result
Immunocytochemistry of HeLa cells, using Histone H2AX Rabbit mAb RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Protein Target Info & Infographic
Gene/Protein Information For H2AFX (Source: Uniprot.org, NCBI)
Gene Name
H2AFX
Full Name
Histone H2AX
Weight
15145 MW
Superfamily
histone H2A family
Alternative Names
Histone H2AX;H2a/x;Histone H2A.X;H2AFX;H2AX; H2AX H2A.X, H2A/X, H2AFX H2A.X variant histone histone H2AX|H2A histone family member X|H2AX histone|histone H2A.x
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2AFX, check out the H2AFX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2AFX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (M00241-2)
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No publications found for M00241-2
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