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Product Info Summary
| SKU: | M02242 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-HIP1 Monoclonal Antibody
SKU/Catalog Number
M02242
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HIP1 Monoclonal Antibody catalog # M02242. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02242)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFG-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02242 is reactive to HIP1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
116 kDa
Calculated molecular weight
25729 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02242 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:30
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human PC-3 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HIP1 using anti-HIP1 antibody (M02242).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP1 antigen affinity purified monoclonal antibody (Catalog # M02242) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP1 at approximately 116 kDa. The expected band size for HIP1 is at 116 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HIP1 (Source: Uniprot.org, NCBI)
Gene Name
HIP1
Full Name
Huntingtin-interacting protein 1
Weight
25729 MW
Superfamily
SLA2 family
Alternative Names
T-cell surface glycoprotein CD8 alpha chain;T-lymphocyte differentiation antigen T8/Leu-2;CD8a;CD8A;MAL; HIP1 HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma huntingtin interacting protein 1 huntingtin-interacting protein 1|huntingtin-interacting protein I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIP1, check out the HIP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIP1 Monoclonal Antibody (M02242)
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No publications found for M02242
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Customer Q&As
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5 Customer Q&As for Anti-HIP1 Monoclonal Antibody
Question
We are currently using anti-HIP1 Monoclonal antibody M02242 for rat tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-HIP1 Monoclonal antibody (M02242) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Is a blocking peptide available for product anti-HIP1 Monoclonal antibody (M02242)?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
We do provide the blocking peptide for product anti-HIP1 Monoclonal antibody (M02242). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-18
Question
Will anti-HIP1 Monoclonal antibody M02242 work for IF with corpus callosum?
C. Roberts
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of corpus callosum, HIP1 is highly expressed in corpus callosum. So, it is likely that anti-HIP1 Monoclonal antibody M02242 will work for IF with corpus callosum.
Boster Scientific Support
Answered: 2019-06-27
Question
Will M02242 anti-HIP1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
As indicated on the product datasheet, M02242 anti-HIP1 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-28
Question
Is this M02242 anti-HIP1 Monoclonal antibody reactive to the isotypes of HIP1?
D. Collins
Verified customer
Asked: 2013-12-06
Answer
The immunogen of M02242 anti-HIP1 Monoclonal antibody is A synthesized peptide derived from human HIP1 Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR) -mediated transcription. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-12-06
