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Product Info Summary
| SKU: | A05145 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Hexokinase-3 HK3 Antibody
View all Hexokinase Type III Antibodies
SKU/Catalog Number
A05145
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Hexokinase-3 HK3 Antibody (Catalog # A05145). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hexokinase-3 HK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05145)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Hexokinase-3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05145 is reactive to HK3 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
99025 MW
Background of Hexokinase Type III
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).
Furuta H., Genomics 36:206-209(1996).
Palma F., Mol. Cell. Biochem. 155:23-29(1996).
Sjoeblom T., Science 314:268-274(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05145 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, treated with insulin (0.01U/ml, 15mins), using Hexokinase-3 antibody A05145.
Protein Target Info & Infographic
Gene/Protein Information For HK3 (Source: Uniprot.org, NCBI)
Gene Name
HK3
Full Name
Hexokinase-3
Weight
99025 MW
Superfamily
hexokinase family
Alternative Names
Hexokinase-3;2.7.1.1;Hexokinase type III;HK III;HK3; HK3 HKIII, HXK3 hexokinase 3 hexokinase-3|ATP:D-hexose 6-phosphotransferase|HK III|hexokinase 3 (white cell)|hexokinase type III|hexokinase-C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HK3, check out the HK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hexokinase-3 HK3 Antibody (A05145)
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No publications found for A05145
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Hexokinase-3 HK3 Antibody
Question
Please see the WB image, lot number and protocol we used for blood using anti-Hexokinase-3 antibody A05145. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
Does A05145 anti-Hexokinase-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Wu
Verified customer
Asked: 2019-01-09
Answer
As indicated on the product datasheet, A05145 anti-Hexokinase-3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-09
Question
I was wanting to use your anti-Hexokinase-3 antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
You can see on the product datasheet, A05145 anti-Hexokinase-3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-01
Question
Is there a BSA free version of anti-Hexokinase-3 antibody A05145 available?
V. Krishna
Verified customer
Asked: 2017-06-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hexokinase-3 antibody A05145 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-14
Question
Is this A05145 anti-Hexokinase-3 antibody reactive to the isotypes of HK3?
B. Carter
Verified customer
Asked: 2016-08-31
Answer
The immunogen of A05145 anti-Hexokinase-3 antibody is Synthesized peptide derived from internal of human Hexokinase-3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-08-31
Question
We are currently using anti-Hexokinase-3 antibody A05145 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
G. Krishna
Verified customer
Asked: 2014-03-17
Answer
The anti-Hexokinase-3 antibody (A05145) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-17
