Anti-Hexokinase-3 HK3 Antibody

Product Name

Anti-Hexokinase-3 HK3 Antibody

View all Hexokinase Type III Antibodies

SKU/Catalog Number

A05145

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Hexokinase-3 HK3 Antibody (Catalog # A05145). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hexokinase-3 HK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05145)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human Hexokinase-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05145 is reactive to HK3 in Human

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

99025 MW

Background of Hexokinase Type III

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

Furuta H., Genomics 36:206-209(1996).
Palma F., Mol. Cell. Biochem. 155:23-29(1996).
Sjoeblom T., Science 314:268-274(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05145 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For HK3 (Source: Uniprot.org, NCBI)

Alternative Names

Hexokinase-3;2.7.1.1;Hexokinase type III;HK III;HK3; HK3 HKIII, HXK3 hexokinase 3 hexokinase-3|ATP:D-hexose 6-phosphotransferase|HK III|hexokinase 3 (white cell)|hexokinase type III|hexokinase-C

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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