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Product Info Summary
| SKU: | A02685-5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-HE4/Wfdc2 Antibody
SKU/Catalog Number
A02685-5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HE4/Wfdc2 Antibody catalog # A02685-5. Tested in ELISA applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HE4/Wfdc2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02685-5)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat HE4 recombinant protein (Position: E31-F168).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02685-5 is reactive to Wfdc2 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated molecular weight
12.993kDa
Background of HE4/WFDC2
WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02685-5 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA Capture (Matched Antibody Pair), 2-8μg/ml
Validation Images & Assay Conditions
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Figure 1. Sandwich ELISA - Recombinant Rat HE4 protein standard curve.
Use in combination with reagents from Rat HE4 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1624).
Protein Target Info & Infographic
Gene/Protein Information For Wfdc2 (Source: Uniprot.org, NCBI)
Gene Name
Wfdc2
Full Name
WAP four-disulfide core domain protein 2
Weight
12.993kDa
Alternative Names
WAP four-disulfide core domain protein 2; Epididymal secretory protein 4; RE4; Wfdc2; WFDC2 EDDM4, HE4, WAP5, dJ461P17.6 WAP four-disulfide core domain 2 WAP four-disulfide core domain protein 2|WAP domain containing protein HE4-V4|epididymal protein 4|epididymal secretory protein E4|epididymis secretory sperm binding protein|epididymis-specific, whey-acidic protein type, four-disulfide core|human epididymis protein 4|major epididymis-specific protein E4|putative protease inhibitor WAP5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Wfdc2, check out the Wfdc2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Wfdc2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HE4/Wfdc2 Antibody (A02685-5)
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