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Product Info Summary
| SKU: | PB9626 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Gremlin 1/GREM1 Antibody Picoband®
SKU/Catalog Number
PB9626
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Gremlin 1/GREM1 Antibody Picoband® catalog # PB9626. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gremlin 1/GREM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9626)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Gremlin 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9626 is reactive to GREM1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
20697 MW
Background of Gremlin 1
Gremlin, also known as Drm, is a highly conserved 20.7-kDa, 184 amino acid glycoprotein part of the DAN family and is a cysteine knot-secreted protein. Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. And Gremlin is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. Gremlin 1 (GREM 1) is known for its antagonistic reaction with BMPs in the TGF beta signaling pathway. This gene inhibits BMP-2, BMP-4, and BMP-7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. Gremlin 1 may play an oncogenic role especially in carcinomas of the uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma. Over-expressed gremlin 1 functions by interaction with YWHAH (Its binding site for gremlin 1 was located between residues 61-80 and gremlin 1 binding site for YWHAH was found to be located between residues 1-67). Therefore, Gremlin 1 and its binding protein YWHAH could be good targets for developing diagnostic and therapeutic strategies against human cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9626 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human A549 whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GREM1 using anti-GREM1 antibody (PB9626).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GREM1 antigen affinity purified polyclonal antibody (Catalog # PB9626) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GREM1 at approximately 21 kDa. The expected band size for GREM1 is at 21 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GREM1 (Source: Uniprot.org, NCBI)
Gene Name
GREM1
Full Name
Gremlin-1
Weight
20697 MW
Superfamily
DAN family
Alternative Names
Gremlin-1;Cell proliferation-inducing gene 2 protein;Cysteine knot superfamily 1, BMP antagonist 1;DAN domain family member 2;Down-regulated in Mos-transformed cells protein;Increased in high glucose protein 2;IHG-2;GREM1;CKTSF1B1, DAND2, DRM;PIG2; Grem1|Cktsf1b, Cktsf1b1, Dr, Drm, Grem, gre, ld|gremlin 1, DAN family BMP antagonist|gremlin-1|cysteine knot superfamily 1, BMP antagonist 1|down-regulated in Mos-transformed cells protein|down-regulated in v-mos-transformed cells
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GREM1, check out the GREM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GREM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gremlin 1/GREM1 Antibody Picoband® (PB9626)
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Customer Q&As
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3 Customer Q&As for Anti-Gremlin 1/GREM1 Antibody Picoband®
Question
We are currently using anti-Gremlin 1/GREM1 antibody PB9626 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
The anti-Gremlin 1/GREM1 antibody (PB9626) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-11
Question
I was wanting to use your anti-Gremlin 1/GREM1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
As indicated on the product datasheet, PB9626 anti-Gremlin 1/GREM1 antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-12
Question
See attached the WB image, lot number and protocol we used for liver using anti-Gremlin 1/GREM1 antibody PB9626. Please let me know if you require anything else.
P. Zhao
Verified customer
Asked: 2015-07-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-31
