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Product Info Summary
| SKU: | A08027-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GRAF/ARHGAP26 Antibody Picoband®
SKU/Catalog Number
A08027-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GRAF/ARHGAP26 Antibody Picoband® catalog # A08027-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GRAF/ARHGAP26 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08027-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GRAF/ARHGAP26 recombinant protein (Position: S584-E791).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08027-1 is reactive to ARHGAP26 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
17136 MW
Background of GRAF
Rho GTPase activating protein 26 (ARHGAP26) also known as GTPase Regulator Associated with Focal Adhesion Kinase (GRAF) is a protein that in humans is encoded by the ARHGAP26 gene. Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08027-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human RT4 whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GRAF/ARHGAP26 using anti-GRAF/ARHGAP26 antibody (A08027-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRAF/ARHGAP26 antigen affinity purified polyclonal antibody (Catalog # A08027-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRAF/ARHGAP26 at approximately 100 kDa. The expected band size for GRAF/ARHGAP26 is at 92 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ARHGAP26 (Source: Uniprot.org, NCBI)
Gene Name
ARHGAP26
Full Name
Rho GTPase-activating protein 26
Weight
17136 MW
Alternative Names
BMP and activin membrane-bound inhibitor homolog; Non-metastatic gene A protein; Putative transmembrane protein; NMA; BAMBI; NMA ARHGAP26 GRAF, GRAF1, OPHN1L, OPHN1L1 Rho GTPase activating protein 26 rho GTPase-activating protein 26|GTPase regulator associated with focal adhesion kinase pp125(FAK)|oligophrenin-1-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGAP26, check out the ARHGAP26 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGAP26: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GRAF/ARHGAP26 Antibody Picoband® (A08027-1)
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