Anti-GPI Antibody (C-term)

Product Name

Anti-GPI Antibody (C-term)

View all Glucose 6 phosphate isomerase Antibodies

SKU/Catalog Number

A00108-2

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-GPI Antibody (C-term) (Catalog # A00108-2). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-GPI Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00108-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00108-2 is reactive to GPI in Human, Mouse

Applications

A00108-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63147 Da

Background of Glucose 6 phosphate isomerase

GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
FC: 1:10-1:50

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody (C-term).A00108-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Click image to see more details

GPI Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Gene/Protein Information For GPI (Source: Uniprot.org, NCBI)

Alternative Names

AMFGNPI; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; glucose phosphate isomerase; glucose-6-phosphate isomerase; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLKSA36; oxoisomerase; PGI; PHI; Phosphoglucose isomerase; phosphohexomutase; Phosphohexose isomerase; phosphosaccharomutase; SA-36; Sperm antigen 36; sperm antigen-36 GPI AMF, GNPI, NLK, PGI, PHI, SA-36, SA36 glucose-6-phosphate isomerase glucose-6-phosphate isomerase|autocrine motility factor|hexose monophosphate isomerase|hexosephosphate isomerase|neuroleukin|oxoisomerase|phosphoglucose isomerase|phosphohexomutase|phosphohexose isomerase|phosphosaccharomutase|sperm antigen-36

For more info on GPI, check out the GPI Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GPI Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis