Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband™ (monoclonal, 6I4E7)

Product Name

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband™ (monoclonal, 6I4E7)

View all Glutathione Peroxidase 4/GPX4 Antibodies

SKU/Catalog Number

M02059-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband™ (monoclonal, 6I4E7) catalog # M02059-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband™ (monoclonal, 6I4E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02059-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

6I4E7

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02059-1 is reactive to GPX4 in Human

Applications

M02059-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

Background of Glutathione Peroxidase 4/GPX4

Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (M02059-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified monoclonal antibody (Catalog # M02059-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.

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Figure 2. Flow Cytometry analysis of Hela cells using anti-Glutathione Peroxidase 4/GPX4 antibody (M02059-1).
Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (M02059-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For GPX4 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.11.1; EC 1.11.1.12; glutathione peroxidase 4 (phospholipid hydroperoxidase); Glutathione Peroxidase 4; GPX4; GPx-4; GSHPx-4; MCSP; PHGPx; PHGPxsnGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; snGPx; snPHGPx; sperm nucleus glutathione peroxidase GPX4 GPx-4, GSHPx-4, MCSP, PHGPx, SMDS, snGPx, snPHGPx glutathione peroxidase 4 phospholipid hydroperoxide glutathione peroxidase|epididymis secretory sperm binding protein|phospholipid hydroperoxidase|phospholipid hydroperoxide glutathione peroxidase, mitochondrial|selenoprotein GPX4|sperm nucleus glutathione peroxidase

For more info on GPX4, check out the GPX4 Infographic

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In this infographic, you will see the following information for GPX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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