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Product Info Summary
| SKU: | A11862-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GLUT6/SLC2A6 Antibody Picoband®
View all Glucose Transporter GLUT6 Antibodies
SKU/Catalog Number
A11862-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GLUT6/SLC2A6 Antibody Picoband® catalog # A11862-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLUT6/SLC2A6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11862-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GLUT6/SLC2A6 recombinant protein (Position: M1-R507).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11862-1 is reactive to SLC2A6 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
54.539kDa
Background of Glucose Transporter GLUT6
Solute carrier family 2, facilitated glucose transporter member 6 is a protein that in humans is encoded by the SLC2A6 gene. It is mapped to 9q34.2. Hexose transport into mammalian cells is catalyzed by a family of membrane proteins, including SLC2A6, that contain 12 transmembrane domains and a number of critical conserved residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11862-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human PC-3 whole cell, rat liver tissue, rat kidney tissue, rat lung tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SLC2A6 using anti-SLC2A6 antibody (A11862-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A6 antigen affinity purified polyclonal antibody (Catalog # A11862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A6 at approximately 55-60KD. The expected band size for SLC2A6 is at 55KD.
Click image to see more details
Figure 2. Western blot analysis of SLC2A6 using anti-SLC2A6 antibody (A11862-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A6 antigen affinity purified polyclonal antibody (Catalog # A11862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A6 at approximately 55-60KD. The expected band size for SLC2A6 is at 55KD.
Protein Target Info & Infographic
Gene/Protein Information For SLC2A6 (Source: Uniprot.org, NCBI)
Gene Name
SLC2A6
Full Name
Solute carrier family 2, facilitated glucose transporter member 6
Weight
54.539kDa
Superfamily
major facilitator superfamily
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 6; Glucose transporter type 6; GLUT-6; Glucose transporter type 9; GLUT-9; SLC2A6; GLUT9 SLC2A6 GLUT6, GLUT9, HSA011372 solute carrier family 2 member 6 solute carrier family 2, facilitated glucose transporter member 6|GLUT-6|GLUT-9|glucose transporter type 6|glucose transporter type 9|solute carrier family 2 (facilitated glucose transporter), member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC2A6, check out the SLC2A6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC2A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLUT6/SLC2A6 Antibody Picoband® (A11862-1)
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Customer Q&As
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6 Customer Q&As for Anti-GLUT6/SLC2A6 Antibody Picoband®
Question
Will A11862-1 anti-GLUT6/SLC2A6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
It shows on the product datasheet, A11862-1 anti-GLUT6/SLC2A6 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
My question regards to test anti-GLUT6/SLC2A6 antibody A11862-1 on rat cervix carcinoma for research purposes, then I may be interested in using anti-GLUT6/SLC2A6 antibody A11862-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-GLUT6/SLC2A6 antibody A11862-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-GLUT6/SLC2A6 antibody A11862-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-22
Question
Is this A11862-1 anti-GLUT6/SLC2A6 antibody reactive to the isotypes of SLC2A6?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The immunogen of A11862-1 anti-GLUT6/SLC2A6 antibody is E.coli-derived human GLUT6/SLC2A6 recombinant protein (Position: M1-R507). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-15
Question
I was wanting to use your anti-GLUT6/SLC2A6 antibody for ELISA for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
You can see on the product datasheet, A11862-1 anti-GLUT6/SLC2A6 antibody has been validated for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-15
Question
We are currently using anti-GLUT6/SLC2A6 antibody A11862-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
T. Banerjee
Verified customer
Asked: 2016-03-28
Answer
The anti-GLUT6/SLC2A6 antibody (A11862-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-28
