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Product Info Summary
| SKU: | A15997 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GK5 Antibody Picoband®
SKU/Catalog Number
A15997
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GK5 Antibody Picoband® catalog # A15997. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GK5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15997)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GK5 recombinant protein (Position: D37-K402). Human GK5 shares 82.2% amino acid (aa) sequence identity with mouse GK5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15997 is reactive to GK5 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
59 kDa
Calculated molecular weight
59 kDa
Background of Gk5
Glycerol kinase 5 (GK5), encoded by the GK5 gene, is a member of the glycerol kinase family involved in glycerol metabolism. While its specific functions are still being elucidated, GK5 is implicated in various physiological processes such as energy metabolism and lipid homeostasis, warranting further investigation into its role and potential implications in health and disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15997 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human A431 whole cell, human SiHa whole cell, human RT4 whole cell
FCM: JK cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GK5 using anti-GK5 antibody (A15997).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GK5 antigen affinity purified polyclonal antibody (Catalog # A15997) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GK5 at approximately 59 kDa. The expected band size for GK5 is at 59 kDa.
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Figure 2. Flow Cytometry analysis of JK cells using anti-GK5 antibody (A15997).
Overlay histogram showing JK cells stained with A15997 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GK5 Antibody (A15997, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GK5 (Source: Uniprot.org, NCBI)
Gene Name
GK5
Full Name
Putative glycerol kinase 5
Weight
59 kDa
Superfamily
FGGY kinase family
Alternative Names
ATP:glycerol 3-phosphotransferase 5; EC 2.7.1.30; FLJ33582; FLJ45739; GK 5; Glycerokinase 5; glycerol kinase 5 (putative); MGC40579; putative glycerol kinase 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GK5, check out the GK5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GK5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GK5 Antibody Picoband® (A15997)
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