Anti-GK5 Antibody Picoband™

Product Name

Anti-GK5 Antibody Picoband™

View all Gk5 Antibodies

SKU/Catalog Number

A15997

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GK5 Antibody Picoband™ catalog # A15997. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GK5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15997)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human GK5 recombinant protein (Position: D37-K402). Human GK5 shares 82.2% amino acid (aa) sequence identity with mouse GK5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A15997 is reactive to Gk5 in Human

Applications

A15997 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

59 kDa

Background of Gk5

Glycerol kinase 5 (GK5), encoded by the GK5 gene, is a member of the glycerol kinase family involved in glycerol metabolism. While its specific functions are still being elucidated, GK5 is implicated in various physiological processes such as energy metabolism and lipid homeostasis, warranting further investigation into its role and potential implications in health and disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GK5 using anti-GK5 antibody (A15997).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GK5 antigen affinity purified polyclonal antibody (Catalog # A15997) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GK5 at approximately 59 kDa. The expected band size for GK5 is at 59 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-GK5 antibody (A15997).
Overlay histogram showing JK cells stained with A15997 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GK5 Antibody (A15997, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Gk5 (Source: Uniprot.org, NCBI)

Alternative Names

ATP:glycerol 3-phosphotransferase 5; EC 2.7.1.30; FLJ33582; FLJ45739; GK 5; Glycerokinase 5; glycerol kinase 5 (putative); MGC40579; putative glycerol kinase 5

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In this infographic, you will see the following information for Gk5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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