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Product Info Summary
| SKU: | A14236 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GIGYF1 Antibody Picoband®
SKU/Catalog Number
A14236
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GIGYF1 Antibody Picoband® catalog # A14236. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GIGYF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14236)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GIGYF1 recombinant protein (Position: K55-E950). Human GIGYF1 shares 89.6% amino acid (aa) sequence identity with mouse GIGYF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14236 is reactive to GIGYF1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
116.238kDa
Background of Gigyf1
This gene encodes a member of the gyf family of adaptor proteins. The encoded protein contains a gyf protein interaction domain. It binds growth factor receptor bound 10, another adaptor protein that binds activated insulin-like growth factor 1 and insulin receptors and regulates receptor signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14236 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human THP-1 whole cell, human Hela whole cell
FCM: SH-SY5Y cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GIGYF1 using anti-GIGYF1 antibody (A14236).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF1 antigen affinity purified polyclonal antibody (Catalog # A14236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF1 at approximately 150 kDa. The expected band size for GIGYF1 is at 115 kDa.
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Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-GIGYF1 antibody (A14236).
Overlay histogram showing SH-SY5Y cells stained with A14236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GIGYF1 Antibody (A14236, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For GIGYF1 (Source: Uniprot.org, NCBI)
Gene Name
GIGYF1
Full Name
GRB10-interacting GYF protein 1
Weight
116.238kDa
Superfamily
GIGYF family
Alternative Names
CDS2; GRB10 interacting GYF protein 1; GRB10-interacting GYF protein 1; GYF domain containing 1; GYF1; PERQ amino acid rich, with GYF domain 1; PERQ amino acid-rich with GYF domain-containing protein 1; PERQ1; postmeiotic segregation increased 2-like 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GIGYF1, check out the GIGYF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GIGYF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GIGYF1 Antibody Picoband® (A14236)
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