Anti-GAMT Antibody Picoband™

Product Name

Anti-GAMT Antibody Picoband™

View all GAMT Antibodies

SKU/Catalog Number

A02886-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-GAMT Antibody Picoband™ catalog # A02886-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GAMT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02886-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human GAMT recombinant protein (Position: M1-G236). Human GAMT shares 88.1% and 86.8% amino acid (aa) sequence identity with mouse and rat GAMT.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02886-2 is reactive to GAMT in Human, Mouse, Rat

Applications

A02886-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

Background of GAMT

Guanidinoacetate N-methyltransferase (EC 2.1.1.2) is an enzyme that catalyzes the chemical reaction and is encoded by gene GAMT located on chromosome 19p13.3. The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GAMT using anti-GAMT antibody (A02886-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAMT antigen affinity purified polyclonal antibody (Catalog # A02886-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAMT at approximately 26 kDa. The expected band size for GAMT is at 26 kDa.

Gene/Protein Information For GAMT (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.1.1.2; guanidinoacetate N-methyltransferase; PIG2; TP53I2 GAMT CCDS2, HEL-S-20, PIG2, TP53I2 guanidinoacetate N-methyltransferase guanidinoacetate N-methyltransferase|epididymis secretory protein Li 20

For more info on GAMT, check out the GAMT Infographic

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In this infographic, you will see the following information for GAMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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