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Product Info Summary
| SKU: | A14343-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GALNT16 Antibody Picoband®
SKU/Catalog Number
A14343-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GALNT16 Antibody Picoband® catalog # A14343-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GALNT16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14343-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GALNT16 recombinant protein (Position: M1-T558). Human GALNT16 shares 96.1% amino acid (aa) sequence identity with mouse GALNT16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14343-1 is reactive to GALNT16 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
63.074kDa
Background of GALNT16
Membrane-bound polypeptide N-acetylgalactosaminyltransferases (EC 2.4.1.41), such as GALNT16, catalyze the first step in mucin-type O-glycosylation of peptides in the Golgi apparatus. These enzymes transfer N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the hydroxyl group of serine or threonine in target proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14343-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GALNT16 using anti-GALNT16 antibody (A14343-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT16 antigen affinity purified polyclonal antibody (Catalog # A14343-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT16 at approximately 70 kDa. The expected band size for GALNT16 is at 63 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GALNT16 (Source: Uniprot.org, NCBI)
Gene Name
GALNT16
Full Name
Polypeptide N-acetylgalactosaminyltransferase 16
Weight
63.074kDa
Superfamily
glycosyltransferase 2 family
Alternative Names
Polypeptide N-acetylgalactosaminyltransferase 16 GALNT16 GALNACT16, GALNTL1, GalNAc-T16 polypeptide N-acetylgalactosaminyltransferase 16 polypeptide N-acetylgalactosaminyltransferase 16|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 1|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 1|galNAc-T-like protein 1|polypeptide GalNAc transferase 16|polypeptide GalNAc transferase-like protein 1|polypeptide N-acetylgalactosaminyltransferase-like protein 1|pp-GaNTase-like protein 1|protein-UDP acetylgalactosaminyltransferase-like protein 1|putative polypeptide N-acetylgalactosaminyltransferase-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALNT16, check out the GALNT16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALNT16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GALNT16 Antibody Picoband® (A14343-1)
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