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Product Info Summary
| SKU: | PB9659 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Galectin 8/LGALS8 Antibody Picoband®
View all Galectin-8 Antibodies
SKU/Catalog Number
PB9659
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Galectin 8/LGALS8 Antibody Picoband® catalog # PB9659. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Galectin 8/LGALS8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9659)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Galectin 8, different from the related mouse and rat sequences by six amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9659 is reactive to LGALS8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
35808 MW
Background of Galectin-8
Galectin-8 is a protein of the galectin family that in humans is encoded by the LGALS8 gene. This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9659 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human COLO-320 whole cell, rat testicular tissue, mouse Neuro-2A whole cell,
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Galectin 8 using anti-Galectin 8 antibody (PB9659).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human COLO-320 whole cell lysates
Lane 2: rat testicular tissue lysates
Lane 3: mouse Neuro-2A whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 8 antigen affinity purified polyclonal antibody (Catalog # PB9659) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 8 at approximately 36KD. The expected band size for Galectin 8 is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For LGALS8 (Source: Uniprot.org, NCBI)
Gene Name
LGALS8
Full Name
Galectin-8
Weight
35808 MW
Alternative Names
Galectin-8;Gal-8;Po66 carbohydrate-binding protein;Po66-CBP;Prostate carcinoma tumor antigen 1;PCTA-1;LGALS8; LGALS8 Gal-8, PCTA-1, PCTA1, Po66-CBP galectin 8 galectin-8|Po66 carbohydrate binding protein|galectin-8g|lectin, galactoside-binding, soluble, 8|po66 carbohydrate-binding protein|prostate carcinoma tumor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LGALS8, check out the LGALS8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LGALS8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Galectin 8/LGALS8 Antibody Picoband® (PB9659)
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Customer Q&As
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4 Customer Q&As for Anti-Galectin 8/LGALS8 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Galectin 8/LGALS8 antibody (PB9659)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-Galectin 8/LGALS8 antibody (PB9659). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-Galectin 8/LGALS8 antibody PB9659 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-01
Question
Does PB9659 anti-Galectin 8/LGALS8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
You can see on the product datasheet, PB9659 anti-Galectin 8/LGALS8 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-29
Question
We are currently using anti-Galectin 8/LGALS8 antibody PB9659 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
L. Singh
Verified customer
Asked: 2015-10-14
Answer
The anti-Galectin 8/LGALS8 antibody (PB9659) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-14
