Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband™

Product Name

Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband™

View all Glucose 6 Phosphate Dehydrogenase Antibodies

SKU/Catalog Number

PB9198

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband™ catalog # PB9198. Tested in WB applications. This antibody reacts with Human;Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9198)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9198 is reactive to G6PD in Human, Mouse

Applications

PB9198 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

59 kDa

Calculated molecular weight

59257 MW

Background of Glucose 6 Phosphate Dehydrogenase

Glucose-6-phosphate dehydrogenase, also known as G6PD or G6PDH, is an enzyme that in humans is encoded by the G6PD gene. It is mapped to Xq28. G6PD plays a key role in the production of ribose 5-phosphate and the generation of NADPH in the hexose monophosphate pathway. Because this pathway is the only NADPH-generation process in mature red cells, which lack the citric adid cycle, a genetic deficiency of G6PD is often associated with adverse physiologic effects. It has been found that aldosterone decreased G6PD expression and activity, resulting in increased oxidant stress and decreased nitric oxide levels, similar to what is observed in G6PD-deficient endothelial cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of G6PD using anti-G6PD antibody (PB9198).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HepG2 whole cell lysates,
Lane 8: human RT4 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified polyclonal antibody (Catalog # PB9198) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa.

Gene/Protein Information For G6PD (Source: Uniprot.org, NCBI)

Alternative Names

G6PD; G6PD1EC 1.1.1.49; G6PDH; glucose-6-phosphate dehydrogenase G6PD G6PD1 glucose-6-phosphate dehydrogenase glucose-6-phosphate 1-dehydrogenase|epididymis secretory sperm binding protein

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