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Product Info Summary
| SKU: | PB9182 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Furin Antibody Picoband®
SKU/Catalog Number
PB9182
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Furin Antibody Picoband® catalog # PB9182. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Furin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9182)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Furin recombinant protein (Position: T591-L794). Human Furin shares 88% amino acid (aa) sequence identity with both mouse and rat Furin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9182 is reactive to FURIN in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
86678 MW
Background of FURIN
Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The Furin gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9182 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human Colo320 whole cell, human SW620 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Furin using anti-Furin antibody (PB9182).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human Furin protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 40 kDa. The expected band size for Furin is at 40 kDa.
Click image to see more details
Figure 2. Western blot analysis of Furin using anti-Furin antibody (PB9182).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Colo320 whole cell lysates,
Lane 4: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 87 kDa. The expected band size for Furin is at 87 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FURIN (Source: Uniprot.org, NCBI)
Gene Name
FURIN
Full Name
Furin
Weight
86678 MW
Superfamily
peptidase S8 family
Alternative Names
Furin;3.4.21.75;Dibasic-processing enzyme;Paired basic amino acid residue-cleaving enzyme;PACE;FURIN;FUR, PACE, PCSK3; FURIN FUR, PACE, PCSK3, SPC1 furin, paired basic amino acid cleaving enzyme furin|FES upstream region|dibasic processing enzyme|furin, membrane associated receptor protein|paired basic amino acid residue-cleaving enzyme|proprotein convertase subtilisin/kexin 3|proprotein convertase subtilisin/kexin type 3|subtilisin-like proprotein convertase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FURIN, check out the FURIN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FURIN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Furin Antibody Picoband® (PB9182)
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Customer Q&As
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5 Customer Q&As for Anti-Furin Antibody Picoband®
Question
Will anti-Furin antibody PB9182 work on feline WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Our lab technicians have not tested anti-Furin antibody PB9182 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Furin antibody PB9182 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-24
Question
Does PB9182 anti-Furin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
It shows on the product datasheet, PB9182 anti-Furin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-Furin antibody PB9182 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-11
Question
The immunogen of this product states it is an E.coli- derived human Furin recombinant protein. Is this a full length or a section of the protein?
Verified Customer
Verified customer
Asked: 2016-09-21
Answer
The immunogen of PB9182 is a section of the recombinant protein. It is located at
Boster Scientific Support
Answered: 2016-09-21
Question
We are currently using anti-Furin antibody PB9182 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
L. Li
Verified customer
Asked: 2013-07-31
Answer
The anti-Furin antibody (PB9182) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-31
