Anti-FREM2 Antibody

Product Name

Anti-FREM2 Antibody

View all Frem2 Antibodies

SKU/Catalog Number

A07915

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FREM2 Antibody (Catalog # A07915). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

FREM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FREM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07915)

Host

Rabbit

Contents

FREM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FREM2 antibody was raised against a 19 amino acid synthetic peptide near the center of human FREM2. The immunogen is located within amino acids 1920 - 1970 of FREM2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms are known to exist. This antibody will only recognize the two longest isoforms.

Reactive Species

A07915 is reactive to Frem2 in Human, Mouse

Applications

A07915 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

351157 MW

Background of Frem2

FREM2 is a member of the FRAS1-related extracellular matrix protein family and like FREM1, mutations in the FREM2 gene are associated with Fraser syndrome. FREM2 localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions during morphogenetic processes and is thought to be required for maintaining the integrity of the skin epithelium and the differentiated state of renal epithelia. The FREM2 gene is one of several genes whose transcription is affecteded by TFAP2C, a transcription factor involved in mammary development, differentiation, and oncogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.

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Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.

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Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.

Gene/Protein Information For Frem2 (Source: Uniprot.org, NCBI)

Alternative Names

FRAS1-related extracellular matrix protein 2 Frem2|6030440P17Rik, 8430406N05Rik, Gm409, b2b1562C, b2b1562Clo, my, n, ne, nv1|Fras1 related extracellular matrix protein 2|FRAS1-related extracellular matrix protein 2|ECM3 homolog|NV domain containing protein NV1|NV domain-containing protein 1

For more info on Frem2, check out the Frem2 Infographic

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In this infographic, you will see the following information for Frem2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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