Anti-FRA2/FOSL2 Antibody Picoband™

Product Name

Anti-FRA2/FOSL2 Antibody Picoband™

View all FRA2 Antibodies

SKU/Catalog Number

PB9591

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FRA2/FOSL2 Antibody Picoband™ catalog # PB9591. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FRA2/FOSL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9591)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FRA2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9591 is reactive to FOSL2 in Human, Rat

Applications

PB9591 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

35193 MW

Background of FRA2

Fos-related antigen 2 is a protein that in humans is encoded by the FOSL2 gene. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FRA2 using anti-FRA2 antibody (PB9591).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Lung Tissue Lysate at 50ug,
Lane 2: HEPG2 Whole Cell Lysate at 40ug,
Lane 3: A549 Whole Cell Lysate at 40ug,
Lane 4: SW620 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA2 antigen affinity purified polyclonal antibody (Catalog # PB9591) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA2 at approximately 43 kDa. The expected band size for FRA2 is at 35 kDa.

Gene/Protein Information For FOSL2 (Source: Uniprot.org, NCBI)

Alternative Names

FOS-like antigen 2; fos-related antigen 2; FRA-2; FRA2FLJ23306 FOSL2 FRA2 FOS like 2, AP-1 transcription factor subunit fos-related antigen 2|FOS like 2, AP-1 trancription factor subunit|FOS like antigen 2|FRA-2

For more info on FOSL2, check out the FOSL2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FOSL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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