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Product Info Summary
| SKU: | M01191 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FGF19 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01191
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FGF19 Rabbit Monoclonal Antibody catalog # M01191. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01191)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24F22
Isotype
IgG
Immunogen
A synthesized peptide derived from human FGF19
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01191 is reactive to FGF19 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
24 kDa
Calculated molecular weight
63888 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01191 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: human SH-SY5Y whole cell, human U-87MG whole cell, human CACO-2 whole cell, human HepG2 whole cell, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (M01191).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified monoclonal antibody (Catalog # M01191) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FGF19 (Source: Uniprot.org, NCBI)
Gene Name
FGF19
Full Name
Fibroblast growth factor 19
Weight
63888 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Cell division cycle 7-related protein kinase;CDC7-related kinase;HsCdc7;huCdc7;2.7.11.1;CDC7;CDC7L1; FGF19 fibroblast growth factor 19 fibroblast growth factor 19|FGF-19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF19, check out the FGF19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF19 Rabbit Monoclonal Antibody (M01191)
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