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Product Info Summary
| SKU: | PA1454 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Fibroblast growth factor 10 FGF10 Antibody Picoband®
SKU/Catalog Number
PA1454
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibroblast growth factor 10 FGF10 Antibody catalog # PA1454. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast growth factor 10 FGF10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1454)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FGF10, different from the related mouse and rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1454 is reactive to FGF10 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23436 MW
Background of FGF-10
FGF 10, Fibroblast growth factor 10, is a protein that in humans is encoded by the FGF10 gene.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF 10 contains 3 exons and spans at least 52.7 kb. By radioactive in situ hybridization, the FGF 10 gene is mapped to 5p13-p12. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts. FGF 10 translocated into urothelial cell nuclei and initiated a signaling cascade that began with the heparin-dependent tyrosine phosphorylation of surface transmembrane receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1454 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: U87 Cell, HELA Cell, A519 Cell, 293T Cell, HELA Cell,
Validation Images & Assay Conditions
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Anti-FGF10 antibody, PA1454, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: A519 Cell Lysate
Lane 4: 293T Cell Lysate
Lane 5: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For FGF10 (Source: Uniprot.org, NCBI)
Gene Name
FGF10
Full Name
Fibroblast growth factor 10
Weight
23436 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10; FGF10 fibroblast growth factor 10 fibroblast growth factor 10|FGF-10|keratinocyte growth factor 2|produced by fibroblasts of urinary bladder lamina propria
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF10, check out the FGF10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibroblast growth factor 10 FGF10 Antibody Picoband® (PA1454)
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No publications found for PA1454
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Customer Q&As
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2 Customer Q&As for Anti-Fibroblast growth factor 10 FGF10 Antibody Picoband®
Question
We are currently using anti-FGF10 antibody PA1454 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The anti-FGF10 antibody (PA1454) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-25
Question
# Please let us know this product is purified by affinity chromatography. # Concentration of sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) if it is contained in the product.
Verified Customer
Verified customer
Asked: 2015-09-30
Answer
PA1454 is purified by affinity chromatography. It contains 0.05mg Thimerosal and 0.05mg NaN3. The concentrations of Thimerosal and NaN3 are both 0.02%.
Boster Scientific Support
Answered: 2015-09-30
