Anti-Factor V/F5 Antibody Picoband®

Product Name

Anti-Factor V/F5 Antibody Picoband®

View all F5 Antibodies

SKU/Catalog Number

A00440-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Factor V/F5 Antibody Picoband® catalog # A00440-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor V/F5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00440-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Factor V.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00440-1 is reactive to F5 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

252 kDa

Calculated molecular weight

251.703kDa

Background of F5

Factor V is a protein of the coagulation system, rarely referred to as proaccelerin or labile factor. The gene for factor V is located on the first chromosome (1q23). This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00440-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Positive Control

WB: human plasma

Validation Images & Assay Conditions

Gene/Protein Information For F5 (Source: Uniprot.org, NCBI)

Alternative Names

Coagulation factor V; Activated protein C cofactor; Proaccelerin, labile factor; Coagulation factor V heavy chain; Coagulation factor V light chain; F5 F5 FVL, PCCF, RPRGL1, THPH2 coagulation factor V coagulation factor V|activated protein c cofactor|coagulation factor V (proaccelerin, labile factor)|coagulation factor V jinjiang A2 domain|factor V Leiden

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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