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Product Info Summary
| SKU: | A04157-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-eRF1/ETF1 Picoband® Antibody
SKU/Catalog Number
A04157-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-eRF1/ETF1 Picoband® Antibody catalog # A04157-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eRF1/ETF1 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04157-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04157-2 is reactive to ETF1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
90314 MW
Background of eRF1
Eukaryotic translation termination factor 1 (eRF1), also known asTB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04157-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Raji whole cell, rat kidney tissue, mouse NIH3T3 whole cell, mouse RAW2467 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ETF1 using anti-ETF1 antibody (A04157-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse NIH3T3 whole cell lysates,
Lane 4: mouse RAW246.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF1 antigen affinity purified polyclonal antibody (Catalog # A04157-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF1 at approximately 49KD. The expected band size for ETF1 is at 49KD.
Protein Target Info & Infographic
Gene/Protein Information For ETF1 (Source: Uniprot.org, NCBI)
Gene Name
ETF1
Full Name
Eukaryotic peptide chain release factor subunit 1
Weight
90314 MW
Superfamily
eukaryotic release factor 1 family
Alternative Names
Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Erf1; Protein Cl1; TB3-1; ETF1; ERF1; RF1; SUP45L1 ETF1 D5S1995, ERF, ERF1, RF1, SUP45L1, TB3-1 eukaryotic translation termination factor 1 eukaryotic peptide chain release factor subunit 1|polypeptide chain release factor 1|protein Cl1|sup45 (yeast omnipotent suppressor 45) homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ETF1, check out the ETF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ETF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eRF1/ETF1 Picoband® Antibody (A04157-2)
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