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Product Info Summary
| SKU: | PB9880 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EPCR/CD201/PROCR Antibody Picoband®
SKU/Catalog Number
PB9880
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPCR/CD201/PROCR Antibody Picoband® catalog # PB9880. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPCR/CD201/PROCR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9880)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human EPCR/CD201 recombinant protein (Position: S18-S210). Human EPCR/CD201 shares 65.5% and 65.6% amino acid (aa) sequence identity with mouse and rat EPCR/CD201, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9880 is reactive to PROCR in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa, 52 kDa
Calculated molecular weight
26671 MW
Background of EPCR
Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor), is a protein that in humans is encoded by the PROCR gene. It has also recently been designated CD201 (cluster of differentiation 201). The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. And it is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9880 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EPCR/CD201 using anti-EPCR/CD201 antibody (PB9880).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCR/CD201 antigen affinity purified polyclonal antibody (Catalog # PB9880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCR/CD201 at approximately 46 kDa, 52 kDa. The expected band size for EPCR/CD201 is at 27 kDa.
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Figure 2. Sandwich ELISA - Recombinant human EPCR protein standard curve.
Use in combination with reagents from Human EPCR ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0957).
Protein Target Info & Infographic
Gene/Protein Information For PROCR (Source: Uniprot.org, NCBI)
Gene Name
PROCR
Full Name
Endothelial protein C receptor
Weight
26671 MW
Alternative Names
Endothelial protein C receptor;Activated protein C receptor;APC receptor;Endothelial cell protein C receptor;CD201;PROCR;EPCR; PROCR CCCA, CCD41, EPCR protein C receptor endothelial protein C receptor|APC receptor|CD201 |activated protein C receptor|cell cycle, centrosome-associated protein|centrocyclin|protein C receptor, endothelial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROCR, check out the PROCR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROCR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EPCR/CD201/PROCR Antibody Picoband® (PB9880)
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Customer Q&As
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3 Customer Q&As for Anti-EPCR/CD201/PROCR Antibody Picoband®
Question
Is there a BSA free version of anti-EPCR/CD201/PROCR antibody PB9880 available?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EPCR/CD201/PROCR antibody PB9880 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-30
Question
Is a blocking peptide available for product anti-EPCR/CD201/PROCR antibody (PB9880)?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We do provide the blocking peptide for product anti-EPCR/CD201/PROCR antibody (PB9880). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-EPCR/CD201/PROCR antibody PB9880 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
The anti-EPCR/CD201/PROCR antibody (PB9880) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01
