Anti-ENTR1 Antibody Picoband™

Product Name

Anti-ENTR1 Antibody Picoband™

View all ENTR1 Antibodies

SKU/Catalog Number

A31716-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ENTR1 Antibody Picoband™ catalog # A31716-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ENTR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31716-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ENTR1 recombinant protein (Position: M1-R424).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A31716-1 is reactive to ENTR1 in Human

Applications

A31716-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

14377 MW

Background of ENTR1

Involved in several processes, including endocytic recycling; positive regulation of cilium assembly; and positive regulation of protein localization to cilium. Located in endosome; microtubule organizing center; and midbody. Colocalizes with retromer complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ENTR1 using anti-ENTR1 antibody (A31716-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENTR1 antigen affinity purified polyclonal antibody (Catalog # A31716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENTR1 at approximately 55 kDa. The expected band size for ENTR1 is at 48 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ENTR1 antibody (A31716-1).
Overlay histogram showing HL-60 cells stained with A31716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENTR1 Antibody (A31716-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For ENTR1 (Source: Uniprot.org, NCBI)

Alternative Names

Endosome-associated-trafficking regulator 1 ENTR1 NY-CO-3, SDCCAG3, SDDAG3 endosome associated trafficking regulator 1 endosome-associated-trafficking regulator 1|antigen NY-CO-3|serologically defined colon cancer antigen 3

For more info on ENTR1, check out the ENTR1 Infographic

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In this infographic, you will see the following information for ENTR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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