Click image to see more details
Product Info Summary
| SKU: | M02833-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)
SKU/Catalog Number
M02833-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) catalog # M02833-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02833-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8B4
Isotype
Mouse IgG2a
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KAT9/ELP3, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02833-1 is reactive to ELP3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.259kDa
Background of ELP3
Elongator complex protein 3, also named KAT9, is aproteinthat in humans is encoded by theELP3gene. It is mapped to 8p21.1. ELP3 is the catalytichistone acetyltransferasesubunit of theRNA polymeraseII elongator complex, which is a component of the RNA polymerase II (Pol II)holoenzymeand is involved in transcriptional elongation. ELP3 supports the migration and branching ofprojection neuronsthroughacetylationof alpha-tubulin in the developingcerebral cortex. In mammals, ELP3 is important for paternalDNA demethylationafterfertilization.ELP3 is potentially involved in cellular redoxhomeostasisby mediating the acetylation ofglucose-6-phosphate dehydrogenase. Besides, ELP3 may play a role inchromatin remodelingand is involved in acetylation ofhistones H3and probablyH4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02833-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Positive Control
WB: human K562 whole cell, human U20S whole cell, human HepG2 whole cell, human U87 whole cell, rat lung tissue, rat PC-12 whole cell, mouse lung tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KAT9/ELP3 using anti-KAT9/ELP3 antibody (M02833-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAT9/ELP3 antigen affinity purified monoclonal antibody (Catalog # M02833-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAT9/ELP3 at approximately 62KD. The expected band size for KAT9/ELP3 is at 62KD.
Protein Target Info & Infographic
Gene/Protein Information For ELP3 (Source: Uniprot.org, NCBI)
Gene Name
ELP3
Full Name
Elongator complex protein 3
Weight
62.259kDa
Superfamily
ELP3 family
Alternative Names
Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6; STFB ELP3 KAT9 elongator acetyltransferase complex subunit 3 elongator complex protein 3|elongation protein 3 homolog|protein lysine acetyltransferase ELP3|tRNA uridine(34) acetyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELP3, check out the ELP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4) (M02833-1)
Hello CJ!
No publications found for M02833-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-KAT9/ELP3 Antibody Picoband® (monoclonal, 8B4)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
