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Product Info Summary
| SKU: | A00861-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband®
View all Cytochrome P450 2B6 Antibodies
SKU/Catalog Number
A00861-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® catalog # A00861-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00861-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Cytochrome P450 2B6/CYP2B6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00861-2 is reactive to CYP2B6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
56.278kDa
Background of Cytochrome P450 2B6
Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. It is mapped to 19q13.2. This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00861-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human Caco-2 whole cell, human HEK293 whole cell, human PC-3 whole cell, human HL-60 whole cell, human K562 whole cell, human A549 whole cell
ICC/IF: U20S cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CYP2B6 using anti-CYP2B6 antibody (A00861-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2B6 antigen affinity purified polyclonal antibody (Catalog # A00861-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2B6 at approximately 56KD. The expected band size for CYP2B6 is at 56KD.
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Figure 2. IF analysis of Cytochrome P450 2B6/CYP2B6 using anti-Cytochrome P450 2B6/CYP2B6antibody (A00861-2).
Cytochrome P450 2B6/CYP2B6was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Cytochrome P450 2B6/CYP2B6 Antibody (A00861-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of THP-1 cells using anti-Cytochrome P450 2B6/CYP2B6 antibody (A00861-2).
Overlay histogram showing THP-1 cells stained with A00861-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 2B6/CYP2B6 Antibody (A00861-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CYP2B6 (Source: Uniprot.org, NCBI)
Gene Name
CYP2B6
Full Name
Cytochrome P450 2B6
Weight
56.278kDa
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2B6; 1,4-cineole 2-exo-monooxygenase; CYPIIB6; Cytochrome P450 IIB1; CYP2B6 CYP2B6 CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, EFVM, IIB1, P450 cytochrome P450 family 2 subfamily B member 6 cytochrome P450 2B6|1,4-cineole 2-exo-monooxygenase|cytochrome P450 IIB1|cytochrome P450, family 2, subfamily B, polypeptide 6|cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2B6, check out the CYP2B6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2B6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband® (A00861-2)
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Customer Q&As
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1 Customer Q&As for Anti-Cytochrome P450 2B6/CYP2B6 Antibody Picoband®
Question
We are currently using anti-Cytochrome P450 2B6/CYP2B6 antibody A00861-2 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
R. Baker
Verified customer
Asked: 2019-11-29
Answer
The anti-Cytochrome P450 2B6/CYP2B6 antibody (A00861-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
