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Product Info Summary
| SKU: | A05286-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CTNNBL1 Antibody Picoband®
SKU/Catalog Number
A05286-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CTNNBL1 Antibody Picoband® catalog # A05286-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CTNNBL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05286-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CTNNBL1 recombinant protein (Position: Q122-E231). Human CTNNBL1 shares 97.3% and 98.2% amino acid (aa) sequence identity with mouse and rat CTNNBL1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05286-1 is reactive to CTNNBL1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
65.173kDa
Background of CTNNBL1
Beta-catenin-like protein 1 is a protein that in humans is encoded by the CTNNBL1 gene. The protein encoded by this gene is a component of the pre-mRNA-processing factor 19-cell division cycle 5-like (PRP19-CDC5L) protein complex, which activates pre-mRNA splicing and is an integral part of the spliceosome. The encoded protein is also a nuclear localization sequence binding protein, and binds to activation-induced deaminase and is important for antibody diversification. This gene may also be associated with the development of obesity. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05286-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CTNNBL1 using anti-CTNNBL1 antibody (A05286-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNBL1 antigen affinity purified polyclonal antibody (A05286-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNBL1 at approximately 65 kDa. The expected band size for CTNNBL1 is at 65 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-CTNNBL1 antibody (A05286-1).
Overlay histogram showing HEL cells stained with A05286-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNBL1 Antibody (A05286-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CTNNBL1 (Source: Uniprot.org, NCBI)
Gene Name
CTNNBL1
Full Name
Beta-catenin-like protein 1
Weight
65.173kDa
Alternative Names
beta-catenin-like protein 1; C20orf33; catenin, beta like 1; dJ633O20.1; FLJ21108; NAPchromosome 20 open reading frame 33; nuclear associated protein; Nuclear-associated protein; NYD-SP19; P14; P14L; PP8304; Testis development protein NYD-SP19 CTNNBL1 C20orf33, NAP, P14L, PP8304, dJ633O20.1 catenin beta like 1 beta-catenin-like protein 1|nuclear associated protein|testis development protein NYD-SP19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTNNBL1, check out the CTNNBL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTNNBL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTNNBL1 Antibody Picoband® (A05286-1)
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