Product Info Summary
SKU: | A16223 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF |
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Product info
Product Name
Anti-Cystatin-like 1 CSTL1 Antibody
SKU/Catalog Number
A16223
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cystatin-like 1 CSTL1 Antibody (Catalog # A16223). Tested in IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystatin-like 1 CSTL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16223)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CSTL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16223 is reactive to CSTL1 in Human
Reconstitution
Calculated molecular weight
16989 MW
Background of CSTL1
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located at the telomeric end of the cystatin locus and encodes a type 2 cystatin-like protein. The specific function of this protein has not been determined.
Deloukas P., Nature 414:865-871(2001).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Xiang Y., Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16223 is guaranteed for IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using CSTL1 antibody A16223.
Protein Target Info & Infographic
Gene/Protein Information For CSTL1 (Source: Uniprot.org, NCBI)
Gene Name
CSTL1
Full Name
Cystatin-like 1
Weight
16989 MW
Superfamily
cystatin family
Alternative Names
Cystatin-like 1;RCET11;CSTL1; CSTL1 CTES1, RCET11, dJ322G13.4 cystatin like 1 cystatin-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSTL1, check out the CSTL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSTL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystatin-like 1 CSTL1 Antibody (A16223)
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No publications found for A16223
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Customer Q&As
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6 Customer Q&As for Anti-Cystatin-like 1 CSTL1 Antibody
Question
Is a blocking peptide available for product anti-CSTL1 antibody (A16223)?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do provide the blocking peptide for product anti-CSTL1 antibody (A16223). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-17
Question
Can you help my question with product A16223, anti-CSTL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16223 anti-CSTL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
I was wanting to use your anti-CSTL1 antibody for IF for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
You can see on the product datasheet, A16223 anti-CSTL1 antibody has been tested for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-23
Question
See below the WB image, lot number and protocol we used for testis using anti-CSTL1 antibody A16223. Please let me know if you require anything else.
L. Krishna
Verified customer
Asked: 2017-09-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-06
Question
Would anti-CSTL1 antibody A16223 work for IF with testis?
P. Walker
Verified customer
Asked: 2017-01-09
Answer
According to the expression profile of testis, CSTL1 is highly expressed in testis. So, it is likely that anti-CSTL1 antibody A16223 will work for IF with testis.
Boster Scientific Support
Answered: 2017-01-09
Question
Do you have a BSA free version of anti-CSTL1 antibody A16223 available?
V. Patel
Verified customer
Asked: 2014-06-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CSTL1 antibody A16223 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-13