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Product Info Summary
| SKU: | A04167-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CRLF2 Antibody Picoband®
View all TSLPR/CRLF2 Antibodies
SKU/Catalog Number
A04167-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CRLF2 Antibody Picoband® catalog # A04167-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRLF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04167-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CRLF2 recombinant protein (Position: A26-K231).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04167-1 is reactive to CRLF2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65-75 kDa
Calculated molecular weight
37.762kDa
Background of TSLPR/CRLF2
Cytokine receptor-like factor 2 is a protein that in humans is encoded by the CRLF2 gene. This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04167-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CRLF2 using anti-CRLF2 antibody (A04167-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human CRLF2 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRLF2 antigen affinity purified polyclonal antibody (Catalog # A04167-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRLF2 at approximately 65-75KD. The expected band size for CRLF2 is at 51KD.
Protein Target Info & Infographic
Gene/Protein Information For CRLF2 (Source: Uniprot.org, NCBI)
Gene Name
CRLF2
Full Name
Cytokine receptor-like factor 2
Weight
37.762kDa
Superfamily
type I cytokine receptor family
Alternative Names
Cytokine receptor-like factor 2; Cytokine receptor-like 2; IL-XR; Thymic stromal lymphopoietin protein receptor; TSLP receptor; CRLF2; CRL2; ILXR; TSLPR Crlf2|CRLM2, Ly11, Ly114, T, Tpte, Tpte2, Tslpr|cytokine receptor-like factor 2|cytokine receptor-like factor 2|TSLP receptor|cytokine receptor-like molecule 2|lymphocyte 114|thymic stromal lymphopoietin protein receptor|thymic stromal-derived lymphopoietin, receptor|transmembrane phosphoinositide 3-phosphatase and tensin homolog 2|type I cytokine receptor delta 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRLF2, check out the CRLF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRLF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CRLF2 Antibody Picoband® (A04167-1)
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Customer Q&As
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1 Customer Q&As for Anti-CRLF2 Antibody Picoband®
Question
We are currently using anti-CRLF2 antibody A04167-1 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
The anti-CRLF2 antibody (A04167-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-28
