Anti-CNOT7 Antibody Picoband™

Product Name

Anti-CNOT7 Antibody Picoband™

View all Cnot7 Antibodies

SKU/Catalog Number

A04884-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-CNOT7 Antibody Picoband™ catalog # A04884-3. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CNOT7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04884-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human CNOT7 recombinant protein (Position: A3-S285).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04884-3 is reactive to Cnot7 in Human, Mouse, Rat

Applications

A04884-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

Background of Cnot7

CCR4-NOT transcription complex subunit 7 is a protein that in humans is encoded by the CNOT7 gene. The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CNOT7 using anti-CNOT7 antibody (A04884-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNOT7 antigen affinity purified polyclonal antibody (Catalog # A04884-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNOT7 at approximately 33 kDa. The expected band size for CNOT7 is at 33 kDa.

Gene/Protein Information For Cnot7 (Source: Uniprot.org, NCBI)

Alternative Names

BTG1 binding factor 1; BTG1-binding factor 1; CAF-1; CAF1carbon catabolite repressor protein (CCR4)-associative factor 1; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 7; CCR4-NOT transcription complex, subunit 7; hCAF-1 Cnot7|AU022737, CAF-1, Caf, Caf1, Pop2|CCR4-NOT transcription complex, subunit 7|CCR4-NOT transcription complex subunit 7|CCR4-associated factor 1|POP2 homolog|catabolite repressor protein (CCR4)-associative factor 1

For more info on Cnot7, check out the Cnot7 Infographic

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In this infographic, you will see the following information for Cnot7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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