Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody

Coagulation Factor X antibody

Boster Bio Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody catalog # A00641. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00641
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody

View all Coagulation Factor X Antibodies

SKU/Catalog Number

A00641

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody catalog # A00641. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00641)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human FA10. AA range:216-265

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00641 is reactive to F10 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

54732 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00641 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For F10 (Source: Uniprot.org, NCBI)

Gene Name

F10

Full Name

Coagulation factor X

Weight

54732 MW

Superfamily

peptidase S1 family

Alternative Names

Coagulation factor X;3.4.21.6;Stuart factor;Stuart-Prower factor;Factor X light chain;Factor X heavy chain;Activated factor Xa heavy chain;F10; F10 FX, FXA coagulation factor X coagulation factor X|Stuart-Prower factor|factor X|factor Xa|prothrombinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on F10, check out the F10 Infographic

F10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for F10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Cleaved-Factor Xa activated HC (I235) F10 Antibody

Question

We are currently using anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

The anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-05

Question

Is a blocking peptide available for product anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641)?

Verified Customer

Verified customer

Asked: 2020-01-07

Answer

We do provide the blocking peptide for product anti-Cleaved-Factor Xa activated HC (I235) antibody (A00641). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-07

Question

I have a question about product A00641, anti-Cleaved-Factor Xa activated HC (I235) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00641 anti-Cleaved-Factor Xa activated HC (I235) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-20

Question

Will anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 work for WB with liver?

Verified Customer

Verified customer

Asked: 2018-12-17

Answer

According to the expression profile of liver, F10 is highly expressed in liver. So, it is likely that anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 will work for WB with liver.

Boster Scientific Support

Answered: 2018-12-17

Question

We want to test anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 on rat liver for research purposes, then I may be interested in using anti-Cleaved-Factor Xa activated HC (I235) antibody A00641 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Li

Verified customer

Asked: 2013-02-15

Answer

The products we sell, including anti-Cleaved-Factor Xa activated HC (I235) antibody A00641, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-02-15

Order DetailsPrice
A00641

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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