Product Info Summary
SKU: | A02720-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-CLC-7 CLCN7 Antibody
SKU/Catalog Number
A02720-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CLC-7 CLCN7 Antibody catalog # A02720-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLC-7 CLCN7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02720-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CLCN7. AA range:10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02720-1 is reactive to CLCN7 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
88679 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02720-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of A549 cells using CLC-7 Polyclonal Antibody diluted at 1:500
Protein Target Info & Infographic
Gene/Protein Information For CLCN7 (Source: Uniprot.org, NCBI)
Gene Name
CLCN7
Full Name
H(+)/Cl(-) exchange transporter 7
Weight
88679 MW
Superfamily
chloride channel (TC 2.A.49) family
Alternative Names
H (+)/Cl (-) exchange transporter 7;Chloride channel 7 alpha subunit;Chloride channel protein 7;ClC-7;CLCN7; CLCN7 CLC-7, CLC7, HOD, OPTA2, OPTB4, PPP1R63 chloride voltage-gated channel 7 H(+)/Cl(-) exchange transporter 7|chloride channel 7 alpha subunit|chloride channel protein 7|chloride channel, voltage-sensitive 7|protein phosphatase 1, regulatory subunit 63
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLCN7, check out the CLCN7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLCN7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLC-7 CLCN7 Antibody (A02720-1)
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No publications found for A02720-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CLC-7 CLCN7 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CLC-7 antibody A02720-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
Would anti-CLC-7 antibody A02720-1 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of placenta, CLCN7 is highly expressed in placenta. So, it is likely that anti-CLC-7 antibody A02720-1 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-CLC-7 antibody A02720-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-CLC-7 antibody (A02720-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01
Question
I see that the anti-CLC-7 antibody A02720-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-23
Question
Would A02720-1 anti-CLC-7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
You can see on the product datasheet, A02720-1 anti-CLC-7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
See attached the WB image, lot number and protocol we used for placenta using anti-CLC-7 antibody A02720-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-17
Question
Can you help my question with product A02720-1, anti-CLC-7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Krishna
Verified customer
Asked: 2017-05-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02720-1 anti-CLC-7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-12