Anti-Centromere protein O CENPO Antibody

Product Name

Anti-Centromere protein O CENPO Antibody

View all Cenpo Antibodies

SKU/Catalog Number

A11021

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Centromere protein O CENPO Antibody (Catalog # A11021). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CENPO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Centromere protein O CENPO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11021)

Host

Rabbit

Contents

CENPO Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO. The immunogen is located within amino acids 170 - 220 of CENPO.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.

Reactive Species

A11021 is reactive to CENPO in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

33786 MW

Background of Cenpo

Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11021 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CENPO (Source: Uniprot.org, NCBI)

Alternative Names

CENP-O, MCM21R, ICEN-36, ICEN36, Centromere protein O, Interphase centromere complex protein 36, CENP-O

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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