Anti-CELSR3/Flamingo Homolog 1 Antibody

Flamingo homolog 1/CELSR3 antibody

Boster Bio Anti-CELSR3/Flamingo Homolog 1 Antibody (Catalog# A07204-1). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07204-1
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC

Product Name

Anti-CELSR3/Flamingo Homolog 1 Antibody

View all Flamingo homolog 1/CELSR3 Antibodies

SKU/Catalog Number

A07204-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CELSR3/Flamingo Homolog 1 Antibody (Catalog# A07204-1). Tested in IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CELSR3/Flamingo Homolog 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07204-1)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CELSR3. Immunogen sequence location: 3195 - 3244

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07204-1 is reactive to CELSR3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

72 kDa

Calculated molecular weight

358185 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A07204-1 is guaranteed for ELISA, IF, ICC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For CELSR3 (Source: Uniprot.org, NCBI)

Gene Name

CELSR3

Full Name

Cadherin EGF LAG seven-pass G-type receptor 3

Weight

358185 MW

Superfamily

G-protein coupled receptor 2 family

Alternative Names

CELSR3;CDHF11;EGFL1;FMI1;KIAA0812;MEGF2;Cadherin EGF LAG seven-pass G-type receptor 3;Cadherin family member 11;Epidermal growth factor-like protein 1;EGF-like protein 1;Flamingo homolog 1;hFmi1;Multiple epidermal growth factor- CELSR3 ADGRC3, CDHF11, EGFL1, FMI1, HFMI1, MEGF2, RESDA1 cadherin EGF LAG seven-pass G-type receptor 3 cadherin EGF LAG seven-pass G-type receptor 3|EGF-like protein 1|EGF-like-domain, multiple 1|adhesion G protein-coupled receptor C3|anchor protein|cadherin family member 11|cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)|epidermal growth factor-like protein 1|flamingo homolog 1|multiple EGF-like domains protein 2|multiple epidermal growth factor-like domains protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CELSR3, check out the CELSR3 Infographic

CELSR3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CELSR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-CELSR3/Flamingo Homolog 1 Antibody

Question

We are currently using anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The anti-CELSR3/Flamingo Homolog 1 antibody (A07204-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-09

Question

I was wanting to use your anti-CELSR3/Flamingo Homolog 1 antibody for IF for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

It shows on the product datasheet, A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody has been validated for IF on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-19

Question

Would A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-01-03

Answer

As indicated on the product datasheet, A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-01-03

Question

Will anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 work for IF with brain?

Verified Customer

Verified customer

Asked: 2017-08-18

Answer

According to the expression profile of brain, CELSR3 is highly expressed in brain. So, it is likely that anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 will work for IF with brain.

Boster Scientific Support

Answered: 2017-08-18

Question

We are interested in to test anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 on rat brain for research purposes, then I may be interested in using anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Miller

Verified customer

Asked: 2013-01-09

Answer

The products we sell, including anti-CELSR3/Flamingo Homolog 1 antibody A07204-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-01-09

Order DetailsPrice
A07204-1

100 μL

$335

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