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Product Info Summary
| SKU: | A07777 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CDCA7 Antibody
SKU/Catalog Number
A07777
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CDCA7 Antibody (Catalog # A07777). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07777)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CDCA7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07777 is reactive to CDCA7 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
42573 MW
Background of Cdca7
Participates in MYC-mediated cell transformation and apoptosis; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. May play a role as transcriptional regulator.
Prescott J.E.,J. Biol. Chem. 276:48276-48284(2001).
Ota T.,Nat. Genet. 36:40-45(2004).
Hillier L.W., Nature 434:724-731(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07777 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using CDCA7 antibody A07777.
Protein Target Info & Infographic
Gene/Protein Information For CDCA7 (Source: Uniprot.org, NCBI)
Gene Name
CDCA7
Full Name
Cell division cycle-associated protein 7
Weight
42573 MW
Alternative Names
Cell division cycle-associated protein 7;Protein JPO1;CDCA7;JPO1; Cdca7|2310021G01Rik, AU044109, JP, JPO1|cell division cycle associated 7|cell division cycle-associated protein 7|c-Myc target JPO1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDCA7, check out the CDCA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDCA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDCA7 Antibody (A07777)
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No publications found for A07777
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CDCA7 Antibody
Question
We are currently using anti-CDCA7 antibody A07777 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-CDCA7 antibody (A07777) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta small intestine using anti-CDCA7 antibody A07777. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-04
Question
Does A07777 anti-CDCA7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
You can see on the product datasheet, A07777 anti-CDCA7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-26
Question
I was wanting to use your anti-CDCA7 antibody for WB for rat placenta small intestine on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat placenta small intestine identification?
W. Gonzalez
Verified customer
Asked: 2016-04-05
Answer
It shows on the product datasheet, A07777 anti-CDCA7 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-05
Question
I was wanting to use to test anti-CDCA7 antibody A07777 on rat placenta small intestine for research purposes, then I may be interested in using anti-CDCA7 antibody A07777 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Bhatt
Verified customer
Asked: 2015-02-02
Answer
The products we sell, including anti-CDCA7 antibody A07777, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-02
Question
I see that the anti-CDCA7 antibody A07777 works with WB, what is the protocol used to produce the result images on the product page?
C. Wu
Verified customer
Asked: 2013-10-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-10-09
