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Product Info Summary
| SKU: | A01188-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD1c Antibody Picoband®
View all CD1c/BDCA-1 Antibodies
SKU/Catalog Number
A01188-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD1c Antibody Picoband® catalog # A01188-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1c Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01188-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD1c recombinant protein (Position: K73-L287).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01188-1 is reactive to CD1C in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
37.654kDa
Background of CD1c/BDCA-1
CD1C is a group-1 member of the CD1 family of major histocompatibility (MHC)-like glycoproteins. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01188-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD1c using anti-CD1c antibody (A01188-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1c antigen affinity purified polyclonal antibody (Catalog # A01188-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1c at approximately 62KD. The expected band size for CD1c is at 38KD.
Protein Target Info & Infographic
Gene/Protein Information For CD1C (Source: Uniprot.org, NCBI)
Gene Name
CD1C
Full Name
T-cell surface glycoprotein CD1c
Weight
37.654kDa
Alternative Names
T-cell surface glycoprotein CD1c; CD1c; CD1C CD1C BDCA1, CD1, CD1A, R7 CD1c molecule T-cell surface glycoprotein CD1c|CD1C , c polypeptide|cortical thymocyte CD1C|differentiation CD1-alpha-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD1C, check out the CD1C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD1C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD1c Antibody Picoband® (A01188-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD1c Antibody Picoband®
Question
My question regards using your anti-CD1c antibody for exogenous lipid antigen via mhc class ib studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
T. Jackson
Verified customer
Asked: 2020-02-14
Answer
Thanks for your inquiry. This A01188-1 anti-CD1c antibody is tested on human placenta tissue. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-14
Question
My boss were satisfied with the WB result of your anti-CD1c antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD1C supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
From literature, leukemic t-cell does express CD1C. Generally CD1C expresses in cell membrane. Regarding which tissues have CD1C expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973
Boster Scientific Support
Answered: 2018-04-18
Question
We have been able to see staining in human thymus. What should we do? Is anti-CD1c antibody supposed to stain thymus positively?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
From literature thymus does express CD1C. From Uniprot.org, CD1C is expressed in thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD1C expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 19349973
Boster Scientific Support
Answered: 2018-02-21
Question
We are currently using anti-CD1c antibody A01188-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
F. Mangal
Verified customer
Asked: 2013-02-05
Answer
The anti-CD1c antibody (A01188-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-05
