Anti-CCDC55 NSRP1 Antibody

Product Name

Anti-CCDC55 NSRP1 Antibody

View all NSRP1 Antibodies

SKU/Catalog Number

A10218

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CCDC55 NSRP1 Antibody (Catalog # A10218). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CCDC55 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CCDC55 NSRP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10218)

Host

Chicken

Contents

CCDC55 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgY

Immunogen

CCDC55 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CCDC55. The immunogen is located within amino acids 430 - 480 of CCDC55.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms are known to exist; this antibody will detect all three.

Reactive Species

A10218 is reactive to NSRP1 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

66390 MW

Background of NSRP1

CCDC55, also known as Nuclear speckle-related protein 70 (NSrp70), was initially identified by the NIH Mammalian Gene Collection. Nuclear speckles are known to be the storage sites of mRNA splicing regulators. CCDC55 has been suggested to modulate alternative pre-mRNA splicing in vivo. It co-localizes and physically interacts with the SR proteins SC35 and ASF/SF2, essential splicing factors which are required for constitutive splicing and can regulate alternative mRNA splicing. Loss of CCDC55 in mice leads to early embryonic lethality highlights the importance of the functional role of CCDC55.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10218 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CCDC55 antibody can be used for detection of CCDC55 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For NSRP1 (Source: Uniprot.org, NCBI)

Alternative Names

CCDC55, NSrp70, HSPC095, CCDC55, NSRP70, Nuclear speckle splicing regulatory protein 1, Coiled-coil domain-containing protein 55 NSRP1 CCDC55, HSPC095, NSrp70 nuclear speckle splicing regulatory protein 1 nuclear speckle splicing regulatory protein 1|coiled-coil domain containing 55|coiled-coil domain-containing protein 55|nuclear speckle-related protein 70

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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