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Product Info Summary
| SKU: | A02392 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-beta 1 Spectrin/SPTB Antibody Picoband®
View all Spectrin beta 1 Antibodies
SKU/Catalog Number
A02392
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-beta 1 Spectrin/SPTB Antibody Picoband® catalog # A02392. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-beta 1 Spectrin/SPTB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02392)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human beta 1 Spectrin/SPTB recombinant protein (Position: M703-Y2137).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02392 is reactive to SPTB in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
270 kDa
Calculated molecular weight
246.468kDa
Background of Spectrin beta 1
Spectrin beta chain, erythrocyte is a protein that in humans is encoded by the SPTB gene. This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02392 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human K562 whole cell, mouse heart tissue, mouse spleen tissue
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of beta 1 Spectrin/SPTB using anti-beta 1 Spectrin/SPTB antibody (A02392).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta 1 Spectrin/SPTB antigen affinity purified polyclonal antibody (Catalog # A02392) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta 1 Spectrin/SPTB at approximately 270 kDa. The expected band size for beta 1 Spectrin/SPTB is at 246 kDa.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-beta 1 Spectrin/SPTB antibody (A02392).
Overlay histogram showing U937 cells stained with A02392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-beta 1 Spectrin/SPTB Antibody (A02392, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SPTB (Source: Uniprot.org, NCBI)
Gene Name
SPTB
Full Name
Spectrin beta chain, erythrocytic
Weight
246.468kDa
Superfamily
spectrin family
Alternative Names
Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12; CASP2; ICH1; NEDD2 SPTB EL3, HS2, HSPTB1, SPH2 spectrin beta, erythrocytic spectrin beta chain, erythrocytic|Sp beta|beta-I spectrin|membrane cytoskeletal protein|spectrin beta Tandil|spectrin beta chain, erythrocyte
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPTB, check out the SPTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta 1 Spectrin/SPTB Antibody Picoband® (A02392)
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