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Product Info Summary
| SKU: | A30517 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ATPBD3 CTU1 Antibody
SKU/Catalog Number
A30517
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATPBD3 CTU1 Antibody (Catalog# A30517). Tested in WB, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATPBD3 CTU1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30517)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ATPBD3. Immunogen sequence location: 291-340
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30517 is reactive to CTU1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
36450 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30517 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from LOVO cells, using ATPBD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CTU1 (Source: Uniprot.org, NCBI)
Gene Name
CTU1
Full Name
Cytoplasmic tRNA 2-thiolation protein 1
Weight
36450 MW
Superfamily
TtcA family
Alternative Names
CTU1;ATPBD3;NCS6;Cytoplasmic tRNA 2-thiolation protein 1;ATP-binding domain-containing protein 3;Cancer-associated gene protein;Cytoplasmic tRNA adenylyltransferase 1 CTU1 ATPBD3, NCS6 cytosolic thiouridylase subunit 1 cytoplasmic tRNA 2-thiolation protein 1|ATP binding domain 3|ATP-binding domain-containing protein 3|cancer-associated gene protein|cancer-associated protein|cytoplasmic tRNA adenylyltransferase 1|cytosolic thiouridylase subunit 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTU1, check out the CTU1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTU1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATPBD3 CTU1 Antibody (A30517)
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Customer Q&As
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7 Customer Q&As for Anti-ATPBD3 CTU1 Antibody
Question
Is a blocking peptide available for product anti-ATPBD3 antibody (A30517)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-ATPBD3 antibody (A30517). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
Does anti-ATPBD3 antibody A30517 work on horse WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Our lab technicians have not validated anti-ATPBD3 antibody A30517 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ATPBD3 antibody A30517 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-27
Question
I have a question about product A30517, anti-ATPBD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30517 anti-ATPBD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ATPBD3 antibody A30517. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
Will A30517 anti-ATPBD3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
As indicated on the product datasheet, A30517 anti-ATPBD3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-18
Question
Do you have a BSA free version of anti-ATPBD3 antibody A30517 available?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ATPBD3 antibody A30517 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-18
Question
We are currently using anti-ATPBD3 antibody A30517 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
R. Banerjee
Verified customer
Asked: 2014-06-30
Answer
The anti-ATPBD3 antibody (A30517) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-30
