Product Info Summary
SKU: | A04927-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ATP6V1B2 Antibody Picoband®
SKU/Catalog Number
A04927-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATP6V1B2 Antibody Picoband® catalog # A04927-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ATP6V1B2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04927-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ATP6V1B2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04927-1 is reactive to ATP6V1B2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
57 kDa
Background of ATP6V1B2
V-type proton ATPase subunit B, brain isoform is an enzyme that in humans is encoded by the ATP6V1B2 gene. This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04927-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human A549 whole cell, human K562 whole cell, human Jurkat whole cell, rat brain tissue, rat H9C2(2-1) whole cell, mouse brain tissue, mouse C2C12 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ATP6V1B2 using anti-ATP6V1B2 antibody (A04927-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat H9C2(2-1) whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B2 antigen affinity purified polyclonal antibody (Catalog # A04927-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1B2 at approximately 57 kDa. The expected band size for ATP6V1B2 is at 57 kDa.
Click image to see more details
Figure 2. Western blot analysis of ATP6V1B2 using anti-ATP6V1B2 antibody (A04927-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B2 antigen affinity purified polyclonal antibody (A04927-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for ATP6V1B2 at approximately 57 kDa. The expected band size for ATP6V1B2 is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ATP6V1B2 (Source: Uniprot.org, NCBI)
Gene Name
ATP6V1B2
Full Name
V-type proton ATPase subunit B, brain isoform
Weight
57 kDa
Superfamily
ATPase alpha/beta chains family
Alternative Names
000 subunit; 56/58kD, isoform 2; ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; Endomembrane proton pump 58 kDa subunit; H+ transporting two-sector ATPase; HO57ATP6B1B2; vacuolar H+-ATPase 56; Vacuolar proton pump subunit B 2; VATB; V-ATPase B2 subunit; V-ATPase subunit B 2; Vma2; VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2; V-type proton ATPase subunit B, brain isoform ATP6V1B2 ATP6B1B2, ATP6B2, DOOD, HO57, VATB, VPP3, Vma2, ZLS2 ATPase H+ transporting V1 subunit B2 V-type proton ATPase subunit B, brain isoform|ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2|H+ transporting two-sector ATPase|V-ATPase B2 subunit|V-ATPase subunit B 2|endomembrane proton pump 58 kDa subunit|testicular secretory protein Li 65|vacuolar H+-ATPase 56,000 subunit|vacuolar proton pump subunit B 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP6V1B2, check out the ATP6V1B2 Infographic
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In this infographic, you will see the following information for ATP6V1B2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP6V1B2 Antibody Picoband® (A04927-1)
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