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Product Info Summary
| SKU: | A03604-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-ARID3A Antibody Picoband®
SKU/Catalog Number
A03604-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARID3A Antibody Picoband® catalog # A03604-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ARID3A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03604-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A, which shares 95.8% amino acid (aa) sequence identity with mouse ARID3A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03604-1 is reactive to ARID3A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
80 kDa
Calculated molecular weight
63 kDa
Background of DRIL1
AT-rich interactive domain-containing protein 3A is a protein that in humans is encoded by the ARID3A gene. This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03604-1 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human HepG2 whole cell
FCM: Caco-2 cells
IP: HepG2 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARID3A using anti-ARID3A antibody (A03604-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID3A antigen affinity purified polyclonal antibody (Catalog # A03604-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID3A at approximately 80 kDa. The expected band size for ARID3A is at 63 kDa.
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Figure2. Flow Cytometry analysis of Caco-2 cells using anti-ARID3A antibody (A03604-1).
Overlay histogram showing Caco-2 cells stained with A03604-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID3A Antibody (A03604-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Immunoprecipitating ARID3A in HepG2 whole cell lysate.
Western blot analysis of ARID3A using anti-ARID3A antibody (A03604-1).
Lane 1: HepG2 whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-ARID3A antibody in HepG2 whole cell lysate,
Lane 3: anti-ARID3A antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ARID3A antigen affinity purified polyclonal antibody (A03604-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ARID3A at approximately 80 kDa. The expected band size for ARID3A is at 63 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ARID3A (Source: Uniprot.org, NCBI)
Gene Name
ARID3A
Full Name
AT-rich interactive domain-containing protein 3A
Weight
63 kDa
Alternative Names
ARID domain-containing 3A; ARID domain-containing protein 3A; AT rich interactive domain 3A (BRIGHT- like) protein; AT rich interactive domain 3A (BRIGHT- like); AT rich interactive domain 3A (BRIGHT-like); AT-rich interactive domain-containing protein 3A; B-cell regulator of IgH transcription; BRIGHT; dead ringer-like 1; Dead ringer-like protein 1; DRIL1dead ringer-like 1 (Drosophila); DRIL3; DRX; E2F-binding protein 1; E2FBP1 ARID3A BRIGHT, DRIL1, DRIL3, E2FBP1 AT-rich interaction domain 3A AT-rich interactive domain-containing protein 3A|ARID domain-containing 3A|ARID domain-containing protein 3A|AT rich interactive domain 3A (BRIGHT- like) protein|AT rich interactive domain 3A (BRIGHT-like)|B-cell regulator of IgH transcription|E2F-binding protein 1|dead ringer-like 1|dead ringer-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARID3A, check out the ARID3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARID3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARID3A Antibody Picoband® (A03604-1)
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