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Product Info Summary
| SKU: | A05317-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-ARHGEF6 Antibody Picoband®
SKU/Catalog Number
A05317-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARHGEF6 Antibody Picoband® catalog # A05317-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ARHGEF6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05317-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human ARHGEF6 recombinant protein (Position: E54-R765). Human ARHGEF6 shares 91.2% and 91.3% amino acid (aa) sequence identity with mouse and rat ARHGEF6, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05317-1 is reactive to ARHGEF6 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
87 kDa
Calculated molecular weight
87 kDa
Background of ARHGEF6
Rho guanine nucleotide exchange factor 6 is a protein that, in humans, is encoded by the ARHGEF6 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05317-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell
FCM: PC-3 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARHGEF6 using anti-ARHGEF6 antibody (A05317-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF6 antigen affinity purified polyclonal antibody (Catalog # A05317-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF6 at approximately 87 kDa. The expected band size for ARHGEF6 is at 87 kDa.
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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-ARHGEF6 antibody (A05317-1).
Overlay histogram showing PC-3 cells stained with A05317-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF6 Antibody (A05317-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF6 (Source: Uniprot.org, NCBI)
Gene Name
ARHGEF6
Full Name
Rho guanine nucleotide exchange factor 6
Weight
87 kDa
Alternative Names
alphaPIX; Alpha-Pix; COOL-2; COOL2alpha; KIAA0006Cool2; mental retardation, X-linked 46; MRX46; PAK-interacting exchange factor alpha; PIXA; Rac/Cdc42 guanine exchange factor (GEF) 6; Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6; Rac/Cdc42 guanine nucleotide exchange factor 6; rho guanine nucleotide exchange factor 6 ARHGEF6 COOL2, Cool-2, MRX46, PIXA, alpha-PIX, alphaPIX Rac/Cdc42 guanine nucleotide exchange factor 6 rho guanine nucleotide exchange factor 6|PAK-interacting exchange factor, alpha|Rac/Cdc42 guanine exchange factor (GEF) 6|Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARHGEF6, check out the ARHGEF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARHGEF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARHGEF6 Antibody Picoband® (A05317-1)
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