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Product Info Summary
| SKU: | P03471-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-APC1 phospho S355 mat-2 Antibody
SKU/Catalog Number
P03471-2
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-APC1 phospho S355 mat-2 Antibody (Catalog # P03471-2). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-APC1 phospho S355 mat-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03471-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P03471-2 is reactive to ANAPC1, TSG24 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
216500 MW
Background of Apc1
APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03471-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:35,000
IP: 1:100
WB: 1:200 - 1:2,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ANAPC1, TSG24 using anti-ANAPC1, TSG24 antibody (P03471-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANAPC1, TSG24 antigen affinity purified polyclonal antibody (Catalog # P03471-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ANAPC1, TSG24.
Protein Target Info & Infographic
Gene/Protein Information For ANAPC1, TSG24 (Source: Uniprot.org, NCBI)
Gene Name
ANAPC1, TSG24
Full Name
Anaphase-promoting complex subunit 1
Weight
216500 MW
Superfamily
APC1 family
Alternative Names
APC1 pS355, Anaphase-promoting complex subunit 1, APC-1, APC 1, Cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator ANAPC1 APC1, MCPR, TSG24 anaphase promoting complex subunit 1 anaphase-promoting complex subunit 1|anaphase-promoting complex 1 (meiotic checkpoint regulator)|cyclosome subunit 1|mitotic checkpoint regulator|testis-specific gene 24 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANAPC1, TSG24, check out the ANAPC1, TSG24 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANAPC1, TSG24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APC1 phospho S355 mat-2 Antibody (P03471-2)
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