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Product Info Summary
| SKU: | PB9125 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Angiopoietin 1/ANGPT1 Antibody Picoband®
View all Angiopoietin-1 Antibodies
SKU/Catalog Number
PB9125
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Angiopoietin 1/ANGPT1 Antibody Picoband® catalog # PB9125. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin 1/ANGPT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9125)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Angiopoietin 1 recombinant protein (Position: H16-N350). Human Angiopoietin 1 shares 96% amino acid (aa) sequence with both mouse and rat Angiopoietin 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9125 is reactive to ANGPT1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
57513 MW
Background of Angiopoietin-1
Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietin 1 is mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9125 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human COLO320 whole cell, human A549 whole cell, human HepG2 whole cell, human 293T whole cell, human SW620 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human Angiopoietin1 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 39 kDa. The expected band size for Angiopoietin 1 is at 39 kDa.
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Figure 2. Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: human SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 65 kDa. The expected band size for Angiopoietin 1 is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ANGPT1 (Source: Uniprot.org, NCBI)
Gene Name
ANGPT1
Full Name
Angiopoietin-1
Weight
57513 MW
Alternative Names
Angiopoietin-1;ANG-1;ANGPT1;KIAA0003; ANGPT1 AGP1, AGPT, ANG1 angiopoietin 1 angiopoietin-1|ANG-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANGPT1, check out the ANGPT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANGPT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiopoietin 1/ANGPT1 Antibody Picoband® (PB9125)
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1 Customer Q&As for Anti-Angiopoietin 1/ANGPT1 Antibody Picoband®
Question
We are currently using anti-Angiopoietin 1/ANGPT1 antibody PB9125 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
The anti-Angiopoietin 1/ANGPT1 antibody (PB9125) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-10
